EarlydevelopmentofprimarymotorneuronsandsomitesinZebrafishEmbryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cleavage in the Zebrafish only occurs inthe blastodisc,a region of cytoplasm in the animal cap of the egg. This type of meroblasticcleavage is called discoidal. Cell divisions are rapid in the zebrafish,with divisions takingabout fifteen minutes each. Gastrulation is usua......閱讀全文
Early－development－of－primary－motor－neurons－and－somites－in－Zebrafish－Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish,?Danio rerio?development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
In－vitroculture－of－early－chick－embryos
1. Use sterile technique. Prewarm Howard's Ringers in petri dish andagar/albumin culture dish to 37oC.2. Crack 2-day egg into large sterile petri
Fate－Mapping－Axolotl－Embryos－in－Early－Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Screening－a－cDNA－Library
Screening a cDNA Libraryfor use with HybriZAP zebrafish cDNA librariesObjectivecDNA library screening allows detection of expressed genes for subseque
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular?Brachydanio rerio?embryos results in
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the embr
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in?Ambystoma mexicanum?embryos. Specifically, we will split the morphogenetic
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
The－Effect－of－pH－Levels－By－Varying－the－Acidity－of－theExtracellular－Solution
BackgroundZebrafish,?Brachydanio rerio, have a distinct embryonic development. The embryo goes through a process?called epiboly. Epiboly, as defined i
Look－Ma,－No－Archenteron!－Sulfates－role－in－sea－urchin－early－development
ObjectiveTo observe the role sulfate plays in sea urchin gastrulation, and to replicate the findings of Karp and Solursh, that sea urchin embryos fail
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
The－Effects－of－NiCl2on－Spicule－Formation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
Zebrafish－whole－mou...
實驗概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is perme
Zebrafish－Staging
In order to repeat a published experiment, or have someone else repeat yours, it is important to use the same materials. For developmental studies, th
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
Fluorescence－Procedures－forthe－ActinandTubulin－Cytoskeleton－inFixed－Cells1
General StrategyWe typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems,
胚胎和成年斑馬魚眼情的組織學準備
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
Observation－of－living－and－plasticembedded－chick－embryos
The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that ther
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula?xenopus?embryos.INTRODUCTION: When th
Fluorescence－Procedures－forthe－Actin－andTubulin－Cytoskeleton－in－Fixed－Cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
在培養脊髓運動神經元過程中所形成由神經元和網絡活...
在培養脊髓運動神經元過程中所形成由神經元和網絡活動構成的網絡這是第一次報告多電極記錄運動神經元網絡。從E15大鼠脊髓腹角分離出來的神經細胞放在Med-64探針里培養。大多數培養的神經元具有神經絲、膽堿乙酰轉移酶和Hb9 等運動神經元的特點。運動神經元網絡的活動特點是具有單個細胞尖峰，并且是自發的
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
BTX特殊電極的應用匯總
BTX特殊電極的應用 此次只將貼壁轉染、活體等進行總結，對于大規模的轉基因的應用，由于BTX公司有新的發展，故此次未將其列入，待下次完善后補充。 1、 Adherent Cell Transfections (ACT)貼壁細胞轉染 ? In-situ electroporat
BTX特殊電極的應用匯總
BTX特殊電極的應用此次只將貼壁轉染、活體等進行總結，對于大規模的轉基因的應用，由于BTX公司有新的發展，故此次未將其列入，待下次完善后補充。1、Adherent Cell Transfections (ACT)貼壁細胞轉染?In-situ electroporation of cells in t
Segmentation－Clock
In most animal species, the anteroposterior body axis is generated by the formation of repeated structures called segments. In vertebrate segmentation